以餐厨垃圾为原料,分别进行批量和半连续厌氧发酵,并对微生物进行Miseq高通量测序,结果表明：在餐厨垃圾批量厌氧发酵情况下,最佳发酵温度为35~40 ℃,最佳发酵浓度约为8%。在餐厨垃圾半连续厌氧发酵情况下,最佳发酵温度约为35 ℃,发酵系统的最佳有机负荷在2.50~3.00 g VS/（L·d）之间。通过高通量测序分析,发现有Synergistaceae、Porphyromonadaceae、Rikenellaceae、Clostridiaceae_1等细菌种群为厌氧发酵系统相对丰度较高的菌群,有Methanosarcinaceae、Methanomicrobiaceae、Methanosaetaceae等古菌种群为厌氧发酵系统相对丰度较高的菌群,但在批量和半连续发酵体系中的含量均有所差别,且不同温度下的优势微生物种群也不尽相同。
Abstract
In this study, kitchen waste was used as raw material to carry out batch fermentation and semi-continuous fermentation, and high-throughput sequencing of microbial species diversity was conducted, and then the research conclusions were drawn. In the batch fermentation of kitchen waste, the optimal fermentation temperature is between 35 ℃ and 40 ℃, and the optimal fermentation concentration is about 8%. In the semi-continuous fermentation of kitchen waste, the optimal fermentation temperature is about 35 ℃, and the optimal organic load of the fermentation system is between 2.50 g VS/（L·d） and 3.00 g VS/（L·d）. Through high-throughput sequencing analysis, it was found that Synergistaceae, Porphyromonadaceae, Rikenellaceae, Clostridiaceae_1 and other bacterial populations had relatively high abundance in the anaerobic fermentation system, while Methanosarcinaceae, Methanomicrobiaceae, Methanosaetaceae and other ancient bacterial populations had relatively high abundance in the anaerobic fermentation system. However, the contents in batch and semi-continuous fermentation systems were different, and the dominant microbial populations were different at different temperatures.
关键词
垃圾处理 /
沼气 /
厌氧消化 /
微生物 /
细菌 /
物种多样性
Key words
garbage disposal /
biogas /
anaerobic digestion /
microorganisms /
bacteria /
species diversity
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