采用爆炸冲击波联合碱处理的方法来提高玉米秸秆的酶解糖化效率。选择爆炸初始压力、碱用量和碱处理温度及时间等主要影响因素,进行单因素和正交试验以获得最优的处理工艺条件。预处理后的秸秆中分别加入2 mg/g（酶/秸秆）的纤维素酶和半纤维素酶,进行酶水解反应5 d,采用高效液相色谱（HPLC）测定其中葡萄糖和木糖浓度从而计算糖化率。结果表明,对糖化率影响程度从大到小依次为碱处理温度、碱用量、碱处理时间和爆炸初始压力。当采用最优实验条件：初始压力550 kPa、碱浓度为8% NaOH、碱处理温度为100 ℃、碱处理时间为60 min,玉米秸秆糖化率可达0.643。爆炸冲击波-碱联合处理玉米秸秆比单纯使用碱处理时,糖化率可提高8%~12%。
Abstract
Shockwave and alkali treatment were combined to enhance the digestibility of corn stover. Initial shock pressure, hydroxide concentration, alkali treatment temperature, and alkali treatment time were investigated to determine their effects on digestibility. Orthogonal experiments were conducted to find the optimal conditions for digestibility. After performing shock and alkali pretreatment adding 2 mg/g （enzyme/straw） cellulase and hemicellulase respectively, the corn stover was enzymatically hydrolyzed for 5 days, and then the sugar concentration were measured with HPLC for calculating the digestibility. The results show that alkali treatment temperature has the greatest effect on digestibility followed by hydroxide concentration, alkali treatment time and initial shock pressure. A maximum digestibility of 0.643 is found under the following conditions： 550 kPa of initial shock pressure, 8% OH-, 100 ℃ of alkali treatment temperature, and 60 minutes of caustic treatment time. The digestibility of corn stover treated with shockwave and alkali can be increased by 8%-12% compared with that treated with alkali alone.
关键词
生物质能 /
爆炸冲击波处理 /
碱预处理 /
酶水解 /
糖化率 /
木质纤维素 /
玉米秸杆
Key words
biomass energy /
shockwave treatment /
alkaline pretreatment /
enzymatic hydrolysis /
digestibility /
lignocellulose /
corn stover
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] DALE B, HOLTZAPPLE M.The need for biofuels[J]. Chemical engineering progress, 2015, 111(3): 36-40, 35.
[2] 杨为华, 李凤和. 常压法秸秆预处理技术研究进展[J].化工进展, 2017, 36(3): 873-880.
YANG W H, LI F H.Research progress on pretreatment techniques of straw at atmospherics pressure[J]. Chemical industry and engineering progress, 2017, 36(3): 873-880.
[3] KUMAR P, BARRETT D M, DELWICHE M J, et al.Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production[J]. Industrial and engineering chemistry research, 2009, 48(8): 3713-3729.
[4] PATRICIA F A, MARCUS B S, FORTER G.Evaluation of the chemical composition of a mixture of sugarcane bagasse and straw after different pretreatments and their effects on commercial enzyme combinations for the production of fermentable sugars[J]. Biomass and bioenergy, 2018, 116: 180-188.
[5] PABLO A, ELIA T P, MERCEDES B, et al.Pretreatment technologies for an efficient bioethanol product on process based on enzymatic hydrolysis:a review[J]. Bioresource technology, 2010, 101(13): 4851-4861.
[6] 熊代余, 汪旭光, 田瑞齐, 等. 木质纤维物料的爆炸冲击裂解预处理新方法[J]. 纤维素科学与技术, 1998, 6(4): 21-27.
XIONG D Y, WANG X G, TIAN R Q, et al.A new method for pretreatment of ligocellulose material by explosive shock[J]. Journal of cellulose science and technology, 1998, 6(4): 21-27.
[7] BOND A, RUGHOONUNDUN H, PEERSEN E, et al.Shock treatment of corn stover[J]. Biotechnol progress, 2017, 33: 815-827.
[8] FALLS M, MADISON M, LIANG C, et al, Mechanical pretreatment of biomass-part II: shock treatment[J]. Biomass and bioenergy, 2019, 126: 47-56.
[9] LIANG C, LONKAR S, DARVEKAR P,et al.Countercurrent enzymatic saccharification of pretreated corn stover. part 2: lime+shock pretreated corn stover and commercial approach[J]. Biomass and bioenergy, 2017, 97: 43-52.
[10] VOCHOZKA M, MAROUSKOVA A.Assessment of shockwave pretreatment in biomass processing[J]. Energy sources, 2017, 39(11): 1195-1199.
基金
国家自然科学基金（11702142）; 校级大学生创新训练项目（2021）; 徐州工程学院校级课题（XKY2013317）
PDF(1540 KB)
