以玉米秸秆为原料,利用本实验室通过基因组重排育种后能高效降解木质纤维素的菌株对玉米秸秆进行预处理,并对预处理后的秸秆进行厌氧沼气发酵。通过正交试验确定复合菌系处理秸秆的最佳条件为：初始pH值为6.00、玉米秸秆含量为2.0%、TR2-35孢子液接种量1.0%、PR2-24孢子液接种量0.5%。通过对秸秆预处理发酵料液的定时检测,确定最佳的预处理时间为8 d,此时发酵液的pH值为6.82,适合厌氧产甲烷菌的生长,发酵液中的COD含量达到最大,为10080 mg/L,秸秆及其成分也达到降解的高峰,适合厌氧沼气发酵。通过设计1 L的厌氧沼气发酵排水集气装置,有效反应体积800 mL,进行20 d沼气发酵,结果表明：预处理组总产气量为6083 mL,总甲烷量为4316 mL,分别较未预处理组提高61.14%和93.63%。
Abstract
Using corn straw as raw material, corn straw was pretreated with the strains that can efficiently degrade lignocellulose after genome rearrangement breeding obtained in our laboratory, and the pretreated straw was fermented by anaerobic biogas. The optimum conditions for the treatment of straw by compound bacteria were determined by orthogonal test as follows： the initial pH value was 6.00, the content of corn straw was 2.0%, the inoculation amount of TR2-35 spore solution was 1.0%, and the inoculation amount of PR2-24 spore solution was 0.5%; the optimal pretreatment time was determined to be 8 d through the regular detection of the fermentation broth of straw pretreatment. At this time, the pH value of the fermentation broth was 6.82, which is suitable for the growth of anaerobic methanogens. The COD content in the fermentation broth reached the maximum value of 10080 mg/L. The straw and its components also reached the peak of degradation, which is suitable for anaerobic biogas fermentation. Through the design of 1 L anaerobic biogas fermentation drainage gas gathering device, the effective reaction volume was 800 mL, and the biogas fermentation was carried out for 20 d. The results show that the total gas production of the pretreatment group is 6083 mL, and the total methane production is 4316 mL, which are 61.14% and 93.63% higher than that of the non-pretreatment group respectively.
关键词
生物质能 /
沼气 /
厌氧发酵 /
复合菌系 /
预处理 /
玉米秸秆
Key words
biomass energy /
biogas /
anaerobic digestion /
compound bacteria /
pretreatment /
corn straw
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参考文献
[1] 高忠坡, 倪嘉波, 李宁宁. 我国农作物秸秆资源量及利用问题研究[J]. 农机化研究, 2022, 44(4): 1-6, 25.
GAO Z P, NI J B, LI N N.Research on the quantity and utilization of crop straw resources in China[J]. Journal of agricultural mechanization research, 2022, 44(4): 1-6,25.
[2] 才金玲, 刘洁, 王乃可, 等. 农作物秸秆综合利用研究进展[J]. 安徽农业科学, 2021, 49(9): 11-14.
CAI J L, LIU J, WANG N K, et al.Research progress on comprehensive utilization of angricultural straw[J]. Journal of Anhui agricultural science, 2021, 49(9): 11-14.
[3] ZHANG K, XU R, ABDMOBRA A E-F, et al. A sustainable approach for efficient conversion of lignin into biodiesel accompanied by biological pretreatment of corn straw[J]. Energy conversion and management, 2019, 199: 111928.
[4] ZHONG C, WANG C M, WANG F X, et al.Enhanced biogas production from wheat straw with the application of synergistic microbial consortium pretreatment[J]. RSC advances, 2016, 6(65): 60187-60195.
[5] HUA B B, DAI J L, LIU B, et al.Pretreatment of non-sterile, rotted silage maize straw by the microbial community MC1 increases biogas production[J]. Bioresource technology, 2016, 216: 699-705.
[6] YADAV M, VIVEKANAND V.Combined fungal and bacterial pretreatment of wheat and pearl millet straw for biogas production: a study from batch to continuous stirred tank reactors[J]. Bioresource technology, 2021, 321: 124523.
[7] 袁旭峰, 高瑞芳, 李培培, 等. 复合菌系MC1预处理对玉米秸秆厌氧发酵产甲烷效率的提高[J]. 农业工程学报, 2011, 27(9): 266-270.
YUAN X F, GAO R F, LI P P, et al.Improvment of anaerobic biogasification efficiency by pretreatment of corn straw with composite microbial system of MC1[J]. Transactions of the Chinese Society of Agricultural Engineering, 2011, 27(9): 266-270.
[8] 潘云霞, 刘思琪, 贺亚清, 等. 复合菌系预处理稻秆半连续厌氧发酵产甲烷性能[J]. 农业工程学报, 2020, 36(11): 261-266.
PAN Y X, LIU S Q, HE Y Q, et al.Methane production performance of semi-continuous anaerobic fermentation with rice straw pretreated by microbial community[J]. Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(11): 261-266.
[9] 任南琪, 王爱杰. 厌氧生物技术原理与应用[M]. 北京: 化学工业出版社, 2004: 313-315.
REN N Q, WANG A J.Principle and application of anaerobic biotechnology[M]. Beijing: Chemical Industry Press, 2004: 313-315.
[10] 郭玉华, 杨伟华, 郁有祝, 等. 蒽酮比色法测定棉花成熟纤维中水溶性总糖含量[J]. 中国棉花, 2011, 38(12): 23-26.
GUO Y H, YANG W H, YU Y Z, et al.Determination of total sugar contents in mature cotton fiber using anthrone colorimetry[J]. China cotton, 2011, 38(12): 23-26.
[11] 于慧娟, 郭夏丽. 秸秆降解菌的筛选及其纤维素降解性能的研究[J]. 生物技术通报, 2019, 35(2): 58-63.
YU H J, GUO X L.Screening of straw-degrading bacteria and study on their cellulose-degrading performances[J]. Biotechnology bulletin, 2019, 35(2): 58-63.
[12] BOOPATHY R, DANIELS L.Effect of pH on anaerobic mild steel corrosion by Methanogenic bacteria[J]. Applied and environmental microbiology, 1991, 57(7): 2104-2108.
[13] LIANG T, ELMAADAWY K, LIU B C, et al.Anaerobic fermentation of waste activated sludge for volatile fatty acid production: recent updates of pretreatment methods and the potential effect of humic and nutrients substances[J]. Process safety and environmental protection, 2021, 145: 321-339.
[14] SAID M, SITANGGANG A S, JULIANDA R, et al.Production of methane as bio-fuel from palm oil mill effluent using anaerobic consortium bacteria[J]. Journal of cleaner production, 2020, 282: 124424.
基金
河南省高等学校重点科研项目（20A180022）; 车用生物燃料技术国家重点实验室开放课题（KFKT2018002）
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