表达漆酶的重组酿酒酵母菌株同步糖化发乙醇特性研究

李娟娟; 王志鹏; 朱士论; 叶禹彤; 路佳琪; 田沈

doi:10.19912/j.0254-0096.tynxb.2022-1781

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太阳能学报 ›› 2024, Vol. 45 ›› Issue (5) : 172-177. DOI: 10.19912/j.0254-0096.tynxb.2022-1781

表达漆酶的重组酿酒酵母菌株同步糖化发乙醇特性研究

李娟娟, 王志鹏, 朱士论, 叶禹彤, 路佳琪, 田沈

作者信息 +

SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE

Li Juanjuan, Wang Zhipeng, Zhu Shilun, Ye Yutong, Lu Jiaqi, Tian Shen

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摘要

将来源于硬毛拴菌（Trametes trogii CICC 2689）的lcc1基因通过Di-CRISPR技术整合到酿酒酵母Y6中以实现漆酶的稳定表达,考察漆酶在酚类抑制剂及同步糖化发酵中的抗逆作用。结果显示：平板拮抗和液体摇瓶实验中,重组菌株Y6/Lac的生长状况、对数期生长速率及延滞期较对照菌株Y6均表现出相应优势;同时Y6/Lac对碱木质素的降解率提高41%,同步糖化发酵产乙醇效率提高14%。

Abstract

Lcc1 gene from Trametes trogii CICC 2689 was integrated into Saccharomyces cerevisiae Y6 by Di-CRISPR to achieve stable expression of laccase, and to investigate the stress resistance of laccase on phenolic inhibitors and simultaneous saccharification and fermentation. The results showed that the recombinant strains Y6/Lac in the medium containing inhibitors had shorter lag phase, higher exponential phase growth rate and final biomass than Y6. At the same time, the degradation rate of alkali lignin by Y6/Lac increased by 41%, and the production efficiency of ethanol by simultaneous saccharification and fermentation increased by 14%.

导出引用

李娟娟, 王志鹏, 朱士论, 叶禹彤, 路佳琪, 田沈. 表达漆酶的重组酿酒酵母菌株同步糖化发乙醇特性研究[J]. 太阳能学报. 2024, 45(5): 172-177 https://doi.org/10.19912/j.0254-0096.tynxb.2022-1781

Li Juanjuan, Wang Zhipeng, Zhu Shilun, Ye Yutong, Lu Jiaqi, Tian Shen. SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE[J]. Acta Energiae Solaris Sinica. 2024, 45(5): 172-177 https://doi.org/10.19912/j.0254-0096.tynxb.2022-1781

中图分类号： TK6

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