采用批式发酵方式,考察菊芋秸秆与猪粪在不同温度（20、25、37 ℃）下共消化特性及关键微生物变化情况。结果表明：不同温度下菊芋秸秆与猪粪共消化时均具有明显的物料协同作用,且共消化能在一定程度上缓解低温导致的甲烷产率低问题。同时,不同温度下共消化的最适配比不同,温度越高最适配比的猪粪含量越高。37 ℃时,菊芋秸秆与猪粪质量配比为1∶4时获得最大积累甲烷产量,为332.29 mL/g VS;而在20和25 ℃时,菊芋秸秆与猪粪配比分别为1∶1和1∶2时最大。微生物分析显示多数优势细菌类群随温度的升高其相对丰度也升高,Syner-01和Chirstensenellaceae-R-7-group等类群在共消化样品中相对丰度较高。温度对古菌群落变化影响显著,随着温度的降低,氢营养型产甲烷菌—产甲烷菌属相对丰度增加;且在不同温度下,共消化系统能够促进不同类型产甲烷菌（甲烷八叠球菌属、甲烷短杆菌属和甲烷杆菌属）的富集。
Abstract
This study employed a batch fermentation method to investigate the characteristics of methane yield and key microbial changes during the co-digestion of Jerusalem artichoke straw and pig manure at various temperatures （of 20, 25 and 37 °C). The findings indicate a significant synergistic effect between Jerusalem artichoke straw and pig manure during co-digestion at different temperatures, effectively mitigating the decreased methane yields typically associated with lower temperatures. However, it was observed that the optimal mixing ratio for co-digestion varied with temperature, with higher temperatures corresponding to higher levels of optimally co-digested pig manure. The maximum cumulative methane yield of 332.29 mL/g VS was achieved when the ratio of Jerusalem artichoke straw to pig manure was 1∶4 at 37 °C, while the maximum methane production was obtained with different mixing ratios of Jerusalem artichoke straw and pig manure were 1∶1 and 1∶2 at 20 and 25 °C. Microbial analysis revealed that the majority of dominant bacterial community increased in relative abundance with increasing temperature, bacterial community such as Syner-01 and Chirstensenellaceae-R-7-group had higher relative abundance in co-digestion samples. Notably, the relative abundance of hydrogenotrophic methanogens, including Methanogenium, increased with decreasing temperature. Furthermore, at different temperature levels, the co-digestion system enriched distinct types of methanogenic archaea, encompassing Methanosarcina, Methanobrevibacter, and Methanobacterium.
关键词
甲烷 /
农业废弃物 /
共消化 /
温度 /
协同作用 /
微生物群落
Key words
methane /
agricultural wastes /
co-digestion /
temperature /
synergy /
microbial community
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基金
青海省自然科学基金面上项目（2023-ZJ-928M）;国家科技部重点研发计划（2022YFD1602400）
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