SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE

Li Juanjuan; Wang Zhipeng; Zhu Shilun; Ye Yutong; Lu Jiaqi; Tian Shen

doi:10.19912/j.0254-0096.tynxb.2022-1781

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Acta Energiae Solaris Sinica ›› 2024, Vol. 45 ›› Issue (5) : 172-177. DOI: 10.19912/j.0254-0096.tynxb.2022-1781

SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE

Li Juanjuan, Wang Zhipeng, Zhu Shilun, Ye Yutong, Lu Jiaqi, Tian Shen

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Abstract

Lcc1 gene from Trametes trogii CICC 2689 was integrated into Saccharomyces cerevisiae Y6 by Di-CRISPR to achieve stable expression of laccase, and to investigate the stress resistance of laccase on phenolic inhibitors and simultaneous saccharification and fermentation. The results showed that the recombinant strains Y6/Lac in the medium containing inhibitors had shorter lag phase, higher exponential phase growth rate and final biomass than Y6. At the same time, the degradation rate of alkali lignin by Y6/Lac increased by 41%, and the production efficiency of ethanol by simultaneous saccharification and fermentation increased by 14%.

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recombinant Saccharomyces cerevisiae / laccase / Di-CRISPR / simultaneous saccharification and fermentation / cellulosic ethanol

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Li Juanjuan, Wang Zhipeng, Zhu Shilun, Ye Yutong, Lu Jiaqi, Tian Shen. SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE[J]. Acta Energiae Solaris Sinica. 2024, 45(5): 172-177 https://doi.org/10.19912/j.0254-0096.tynxb.2022-1781

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