COHESIN-DOCKERIN ASSEMBLY INTERACTIONS MECHANISM OF CBP DESIGNER CELLULOSOME

Xu Shi; Wan Ping; Li Juanjuan; Liu Han; Du Jiliang; Tian Shen

doi:10.19912/j.0254-0096.tynxb.2022-1782

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Acta Energiae Solaris Sinica ›› 2024, Vol. 45 ›› Issue (3) : 139-144. DOI: 10.19912/j.0254-0096.tynxb.2022-1782

COHESIN-DOCKERIN ASSEMBLY INTERACTIONS MECHANISM OF CBP DESIGNER CELLULOSOME

Xu Shi, Wan Ping, Li Juanjuan, Liu Han, Du Jiliang, Tian Shen

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Abstract

In order to explore the molecular interaction between recombinant protein cohesin module and dockerin module and its effect on the assembly efficiency. In this study, scaffoldins and enzymes was secreted by Saccharomyces cerevisiae cells, and the interaction of secondary scaffolins and cellulases combined with primary scaffolin was determined and analyzed by non-denatured electrophoresis and isothermal titration calorimetry in vitro. The results showed that the affinity force between the dockerin module of secondary scaffold protein and cohesion module of cellulase protein decreased while the affinity constant (K_d) increased. The assembly reaction was driven by enthalpy change and hydrogen bonds are generated. The results proved that the decrease of intermolecular affinity of the proteins was the main factor that leads to the lower assembly efficiency of secondary scaffold proteins and primary scaffold proteins.

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Saccharomyces cerevisiae / designer cellulosome / cellulase / self-assembly / protein mutual recognition / molecular dynamic

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Xu Shi, Wan Ping, Li Juanjuan, Liu Han, Du Jiliang, Tian Shen. COHESIN-DOCKERIN ASSEMBLY INTERACTIONS MECHANISM OF CBP DESIGNER CELLULOSOME[J]. Acta Energiae Solaris Sinica. 2024, 45(3): 139-144 https://doi.org/10.19912/j.0254-0096.tynxb.2022-1782

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