EFFECT OF TEMPERATURE ON CO-DIGESTION CHARACTERISTICS AND MICROBIAL COMMUNITY OF Jerusalem artichoke STRAW AND PIG MANURE

Wang Wei; Li Yi; Du Zhongping; Han Rui

doi:10.19912/j.0254-0096.tynxb.2023-1488

PDF(1667 KB)

Acta Energiae Solaris Sinica ›› 2024, Vol. 45 ›› Issue (11) : 528-535. DOI: 10.19912/j.0254-0096.tynxb.2023-1488

EFFECT OF TEMPERATURE ON CO-DIGESTION CHARACTERISTICS AND MICROBIAL COMMUNITY OF Jerusalem artichoke STRAW AND PIG MANURE

Wang Wei, Li Yi, Du Zhongping, Han Rui

Author information +

History +

Abstract

This study employed a batch fermentation method to investigate the characteristics of methane yield and key microbial changes during the co-digestion of Jerusalem artichoke straw and pig manure at various temperatures （of 20, 25 and 37 °C). The findings indicate a significant synergistic effect between Jerusalem artichoke straw and pig manure during co-digestion at different temperatures, effectively mitigating the decreased methane yields typically associated with lower temperatures. However, it was observed that the optimal mixing ratio for co-digestion varied with temperature, with higher temperatures corresponding to higher levels of optimally co-digested pig manure. The maximum cumulative methane yield of 332.29 mL/g VS was achieved when the ratio of Jerusalem artichoke straw to pig manure was 1∶4 at 37 °C, while the maximum methane production was obtained with different mixing ratios of Jerusalem artichoke straw and pig manure were 1∶1 and 1∶2 at 20 and 25 °C. Microbial analysis revealed that the majority of dominant bacterial community increased in relative abundance with increasing temperature, bacterial community such as Syner-01 and Chirstensenellaceae-R-7-group had higher relative abundance in co-digestion samples. Notably, the relative abundance of hydrogenotrophic methanogens, including Methanogenium, increased with decreasing temperature. Furthermore, at different temperature levels, the co-digestion system enriched distinct types of methanogenic archaea, encompassing Methanosarcina, Methanobrevibacter, and Methanobacterium.

Key words

methane / agricultural wastes / co-digestion / temperature / synergy / microbial community

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Wang Wei, Li Yi, Du Zhongping, Han Rui. EFFECT OF TEMPERATURE ON CO-DIGESTION CHARACTERISTICS AND MICROBIAL COMMUNITY OF Jerusalem artichoke STRAW AND PIG MANURE[J]. Acta Energiae Solaris Sinica. 2024, 45(11): 528-535 https://doi.org/10.19912/j.0254-0096.tynxb.2023-1488

References

[1] LI H, DAI M W, DAI S L, et al.Current status and environment impact of direct straw return in China’s cropland: a review[J]. Ecotoxicology and environmental safety, 2018, 159: 293-300.
[2] CICCOLI R, SPERANDEI M, PETRAZZUOLO F, et al.Anaerobic digestion of the above ground biomass of Jerusalem Artichoke in a pilot plant: impact of the preservation method on the biogas yield and microbial community[J]. Biomass and bioenergy, 2018, 108: 190-197.
[3] ZHAO W Y, DENG J B, CHI S L, et al.Sustainability assessment of topsoil ecology in Chongqing, China based on the application of livestock and poultry manure[J]. Journal of cleaner production, 2022, 358: 131969.
[4] 任元森, 张海波, 程红艳, 等. pH值对猪粪与菌糠共发酵产酸特性及微生物群落的影响[J]. 河南农业科学, 2023, 52(5): 110-120.
REN Y S, ZHANG H B, CHENG H Y, et al.Effect of pH value on acid production characteristics and microbial community in co-fermentation of pig manure and spent mushroom substrate[J]. Journal of Henan agricultural sciences, 2023, 52(5): 110-120.
[5] 袁月祥, 廖银章, 刘晓风, 等. 玉米秸秆与猪粪混合发酵产沼气及其细菌解析[J]. 太阳能学报, 2015, 36(8): 1971-1977.
YUAN Y X, LIAO Y Z, LIU X F, et al.Biogas production and its bacteria community during co-fermentation of pig manure and corn stalks[J]. Acta energiae solaris sinica, 2015, 36(8): 1971-1977.
[6] 任海伟, 姚兴泉, 李金平, 等. TS对青贮玉米秸秆与牛粪混合消化产气特性影响[J]. 太阳能学报, 2019, 40(4): 1085-1092.
REN H W, YAO X Q, LI J P, et al.Effect of total solid concentration on biogas production performance during anaerobic co-digestion of maize silages and cattle manure[J]. Acta energiae solaris sinica, 2019, 40(4): 1085-1092.
[7] MAO C L, FENG Y Z, WANG X J, et al.Review on research achievements of biogas from anaerobic digestion[J]. Renewable and sustainable energy reviews, 2015, 45: 540-555.
[8] WANG S W, MA F, MA W W, et al.Influence of temperature on biogas production efficiency and microbial community in a two-phase anaerobic digestion system[J]. Water, 2019, 11(1): 133.
[9] ALROWAIS R, SAID N, AL-OTAIBI A, et al.Comparing the effect of mesophilic and thermophilic anaerobic co-digestion for sustainable biogas production: an experimental and recurrent neural network model study[J]. Journal of cleaner production, 2023, 392: 136248.
[10] LI D J, SONG L Y, FANG H L, et al.Effect of temperature on the anaerobic digestion of cardboard with waste yeast added: dose-response kinetic assays, temperature coefficient and microbial co-metabolism[J]. Journal of cleaner production, 2020, 275: 122949.
[11] 杨斌,殷引,张浩博,等.洗涤剂法测定烟草及烟草制品中中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素的研究[J].中国烟草学报, 2012, 18(3):10-15.
YANG B, YIN Y, ZHANG B H, et al.Determination of NDF, ADF and ADL in tobacco and tobacco products with detergent method[J]. Acta tabacaria sinica, 2012, 18(3): 10-15.
[12] 任海伟, 王宇杰, 李金平, 等. 温度对蔬菜垃圾与猪粪混合消化产沼气特性的影响[J]. 太阳能学报, 2018, 39(8): 2088-2095.
REN H W, WANG Y J, LI J P, et al.Effect of temperature on biogas production from anaerobic co-digestion of vegetable waste and swine manure[J]. Acta energiae solaris sinica, 2018, 39(8): 2088-2095.
[13] KARKI R, CHUENCHART W, SURENDRA K C, et al.Anaerobic co-digestion of various organic wastes:kinetic modeling and synergistic impact evaluation[J]. Bioresource technology, 2022, 343: 126063.
[14] CAO L P, KEENER H, HUANG Z H, et al.Effects of temperature and inoculation ratio on methane production and nutrient solubility of swine manure anaerobic digestion[J]. Bioresource technology, 2020, 299: 122552.
[15] 许继飞, 张秋萍, 朱天骄, 等. 温度和搅拌对牛粪厌氧消化系统抗生素抗性基因变化和微生物群落的影响[J]. 环境科学, 2021, 42(6): 2992-2999.
XU J F, ZHANG Q P, ZHU T J, et al.Effects of temperature and stirring on the changes of antibiotic resistance genes and microbial communities in anaerobic digestion of dairy manure[J]. Environmental science, 2021, 42(6): 2992-2999.
[16] CHEN S, CHENG H C, WYCKOFF K N, et al.Linkages of firmicutes and bacteroidetes populations to methanogenic process performance[J]. Journal of industrial microbiology & biotechnology, 2016, 43(6): 771-781.
[17] LI D, LIU S C, MI L, et al.Effects of feedstock ratio and organic loading rate on the anaerobic mesophilic co-digestion of rice straw and pig manure[J]. Bioresource technology, 2015, 187: 120-127.
[18] 胡进会, 孟伟, 夏怡, 等. 生物-非生物添加剂协同促进餐饮业厨余垃圾厌氧消化: 产气效能及微生物菌群动态变化[J]. 中国环境科学, 2023, 43(4): 1757-1764.
HU J H, MENG W, XIA Y, et al.A synergistic promotion during anaerobic digestion of food waste generated from catering industry by the bio-abiotic additives—biogas production efficiency and corresponding microbial dynamic changes[J]. China environmental science, 2023, 43(4): 1757-1764.
[19] DONG L L, CAO G L, WU J W, et al.Reflux of acidizing fluid for enhancing biomethane production from cattle manure in plug flow reactor[J]. Bioresource technology, 2019, 284: 248-255.
[20] ZHANG J Y, WANG Z Y, LU T D, et al.Response and mechanisms of the performance and fate of antibiotic resistance genes to nano-magnetite during anaerobic digestion of swine manure[J]. Journal of hazardous materials, 2019, 366: 192-201.
[21] 孔德望, 张克强, 房芳, 等. 猪粪厌氧发酵消化液回流体系微生物群落结构特征与产气关系研究[J]. 农业环境科学学报, 2018, 37(3): 559-566.
KONG D W, ZHANG K Q, FANG F, et al.Study of microbial community and biogas production in anaerobic digestion of pig manure with digested slurry recirculation[J]. Journal of agro-environment science, 2018, 37(3): 559-566.
[22] 韩睿, 朱德锐, 李屹, 等. 发酵温度对青海农用沼气池微生物群落的影响[J]. 中国环境科学, 2019, 39(6): 2483-2494.
HAN R, ZHU D R, LI Y, et al.The effect of temperature on the microbial community of rural household biogas digesters in Qinghai Province[J]. China environmental science, 2019, 39(6): 2483-2494.
[23] 张立国, 班巧英, 李建政. UASB反应器中产甲烷菌对温度胁迫的响应[J]. 中国环境科学, 2016, 36(4): 1082-1086.
ZHANG L G, BAN Q Y, LI J Z.Response of methanogens on temperature stress in an UASB reactor[J]. China environmental science, 2016, 36(4): 1082-1086.
[24] 鲁起顺. 沼液回流对秸秆厌氧发酵碳氮形态转化及菌群结构的影响[D]. 沈阳: 沈阳农业大学, 2019.
LU Q S.Effects of biogas slurry reflux on carbon and nitrogen speciation transformation and microbial community structure of straw anaerobic fermentation[D]. Shenyang: Shenyang Agricultural University, 2019.