STUDY ON EFFECT OF ALKALINE HYDROGEN PEROXIDE PRETREATMENT ON ENZYMATIC HYDROLYSIS OF REED STRAW

Li Yu; Xu Zhongbin; Wang Zhongming; Zou Qinqin; Sun Yaguang; Chen Yang

doi:10.19912/j.0254-0096.tynxb.2024-0814

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Acta Energiae Solaris Sinica ›› 2025, Vol. 46 ›› Issue (9) : 208-216. DOI: 10.19912/j.0254-0096.tynxb.2024-0814

STUDY ON EFFECT OF ALKALINE HYDROGEN PEROXIDE PRETREATMENT ON ENZYMATIC HYDROLYSIS OF REED STRAW

Li Yu^1,2, Xu Zhongbin², Wang Zhongming², Zou Qinqin^1,2, Sun Yaguang¹, Chen Yang²

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Abstract

Reed straw was subjected to pretreatment using alkaline hydrogen peroxide under atmospheric pressure and low-temperature conditions. Single-factor experiments were conducted to investigate the effects of varying NaOH concentration, H₂O₂ addition amount, time and temperature. Response surface optimization experiments were subsequently performed, yilding the following optimal parameters： 1.38% NaOH, 1.67% H₂O₂, 2.17 hours, and 69 °C. Under these conditions, the lignin removal rate reached 83.83%, with cellulose and hemicellulose retention rates of 91.69% and 61.94%, respectively. The enzymatic hydrolysis of the pretreated residue achieved a 24-hour hydrolysis rate of 71.08%, significantly surpassing that of the untreated raw material under equivalent conditions, reaching enzymatic equilibrium. Analytical techniques, including Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and scanning electron microscopy, were employed to analyze both raw materials and pretreatment residues. The results demonstrated that the alkaline hydrogen peroxide pretreatment effectively removed lignin and hemicellulose, disrupted the structural integrity of the raw material, and enhanced the efficiency of enzymatic hydrolysis for sugar production. These findings offer valuable technical insights into the high value-added utilization of reed straw.

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biomass / alkaline hydrogen peroxide / enzymolysis / pretreatment / lignin removal rate

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Li Yu, Xu Zhongbin, Wang Zhongming, Zou Qinqin, Sun Yaguang, Chen Yang. STUDY ON EFFECT OF ALKALINE HYDROGEN PEROXIDE PRETREATMENT ON ENZYMATIC HYDROLYSIS OF REED STRAW[J]. Acta Energiae Solaris Sinica. 2025, 46(9): 208-216 https://doi.org/10.19912/j.0254-0096.tynxb.2024-0814

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