该文主要研究不同有机负荷发酵条件下废水的毒性作用规律,并考察三维电解技术的解毒效果。实验结果表明,随着有机负荷的升高,产甲烷活性抑制程度逐渐增强且COD去除率急剧降低,在4 g COD/L时产甲烷抑制率达到38.2%。结合高通量测序技术对发酵过程中细菌和古菌进行分析,发现微生物菌群结构发酵前后变化较为明显。而经优化的三维电解条件（电解时间90 min,电压5 V,pH=6,铁炭添加量140 g/L）对木醋液进行预处理后,4 g COD/L木醋液的甲烷产率由223.1 mL/g COD提升至344.2 mL/g COD,而抑制率降至11.2%。三维电解对于改善木醋液的可生化性及提高厌氧发酵效率有较为明显的效果。
Abstract
In this experiment, the toxicity of wastewater under different organic loads is studied, and the detoxification of three-dimensional electrolysis technology is investigated. The experimental results show that with the increase of organic loads, the inhibitory degree of methanogenic activity is gradually enhanced and the COD removal rate is sharply reduced. The inhibition rate of methanogenic process reaches 38.2% at 4 g COD/L. Combining with the high-throughput sequencing technology, the analysis of bacteria and archaea during fermentation reveals that the microbial community structure is significantly affected before and after fermentation. After the pretreatment of wood vinegar by the optimized three-dimensional electrolysis conditions （electrolysis time 90 min, voltage 5 V, pH=6, iron and carbon concentration 140 g/L）, the methane yield at 4 g COD/L of wood vinegar increases from 223.1 mL/g COD to 344.2 mL/g COD, and the inhibition rate decreased to 11.2%. In summary, three-dimensional electrolysis has an obvious effect on improving the bioavailability and anaerobic fermentation efficiency of wood vinegar.
关键词
厌氧发酵 /
毒性作用 /
废水处理 /
木醋液 /
三维电解 /
微生物菌群
Key words
anaerobic digestion /
toxicity /
wastewater treatment /
wood vinegar /
three-dimensional electrolysis /
microbial community
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基金
山东省高等学校青年创新团队项目（2019KJD002）; 济南市科技计划（201913013）; 山东省生物工程技术创新中心重大创新项目（2019JSWGCCXZX002）; 山东省自然科学基金（ZR2018LB031）
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