在最优高温液态水预处理条件（180 ℃,40 min,固液比1∶20,饱和蒸汽压）下,比较3种不同能源草作物的表现,其中杂交狼尾草Ⅰ号、Ⅱ号和柳枝稷的木糖收率分别为88.46%、98.09%、83.65%。3种能源草经高温液态水水解后酶解率增大,最高能接近100%,总糖收率约为90%。3种能源草后续酶解表现也有差异,柳枝稷预处理前后酶解率增加约50%,狼尾草Ⅱ号和Ⅰ号分别约为40%和20%。通过组分分析,酶解率增大可能源于木质素和半纤维素的降解和纤维素的裸露导致纤维素酶更易与底物接触,木质素的脱除程度的差异使3种原料酶解率的增幅不同。
Abstract
Under the optimal high temperature liquid water （HTLW） pretreatment conditions （180 ℃, 40 min, solid-liquid ratio of 5%, the saturation vapor pressure）, the performance of three different energy grasses are compared. The xylose yields of hybrid Pennisetum Ⅰ, Ⅱ and switchgrass were 88.46%, 98.09%, 83.65% respectively. The enzymatic hydrolysis rates of the three energy grasses were increased after high temperature liquid water hydrolysis, the maximum enzymolysis rate was close to 100%, total sugar yield was about 90%, so to some extent high temperature liquid water pretreatment can be seen to increase the sugar recovery. In addition, there are also differences in the subsequent hydrolysis performance of the three grasses. The enzymatic hydrolysis rate of switchgrass was increased by about 50% before and after pretreatment, while Pennisetum Ⅱ, Ⅰ was increased about 40% and 20%, respectively. Through composition analysis, the increase of enzymatic hydrolysis rate may be caused by the degradation of lignin and hemicellulose and the exposure of cellulosewhich leads to easier contact between cellulase and substrate. The difference of lignin removal degree resulted in different increase of enzymatic hydrolysis rate of the three raw materials
关键词
生物质 /
酶水解 /
生物乙醇 /
能源草 /
高温液态水
Key words
biomass /
enzymatic hydrolysis /
bioethanol /
energy grasses /
high temperature liquid water （HTLW）
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基金
国家自然科学基金（51976221; 51876206; 51606203）; 中科院先导专项（XDA21050400）; 国家重点研发计划（2018YFB1501402）; 广州市科技计划（201803030007）
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